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Home / Mushroom Cultivation / Mushroom Grow Kits / MonoBox Grow Kit

Sterile grow kit A colonised MonoBox cake held in one hand with mushrooms fruiting from every surface 1 / 11
Show: A colonised MonoBox cake held in one hand with mushrooms fruiting from every surface
Show: Mushrooms fruiting inside a sealed grow bag, condensation on the plastic
Show: A hand holding a MonoBox cake covered in mushrooms fruiting from all sides, on a white background
Show: Illustration of a grow bag fruiting: mushrooms emerging from colonised grain inside a filter bag
Show: Grain heavily colonised with white fluffy mycelium filling the jar
Show: Mixing broken grain spawn through with fingers inside the tub
Show: Several sealed green Clinell sachets of 2% chlorhexidine in 70% alcohol wipes
Show: Several sealed blue Clinell antibacterial hand wipe sachets fanned out
Show: A blue disposable pleated face mask with ear loops on a white background
Show: Two sterile 16 gauge 40mm needles in sealed clear packaging
Show: A MonoBox substrate tub: clear tub of dark coir with two green tabs on the lid

Real cultivation

MonoBox Grow Kit

Updated for 2026 with a bigger SpawnTub, custom Cylo Grow Tent, massive humidity and new substrate recipe. Ready to inject with your favourite gourmet mushroom spore or culture syringes. The new fruiting tent can deliver results on par with the CyLab!

Original price was: £40.00.Current price is: £30.00.

Made to order - usually dispatched same or next working day when paid by 1pm.

Sterile and ready-to-inject with a spore or culture syringe of your favourite gourmet mushroom species.

Made in the UK
filled and sterilised fresh to order
Sterile guarantee
arrives sterile or we replace it
You inoculate it
any gourmet species, your choice

Meet the MonoBox grow kit

You can harvest plenty of fresh mushrooms to share with friends and family with our MonoBox grow kit. You'll first inoculate grain and wait for it to colonise, giving you spawn. Then you'll prepare a substrate and combine both together in your MonoBox. When the mushroom mycelium takes over this, you're ready to fruit and enjoy loads of gourmet mushrooms!

If you're used to using our all-in-one kits, you'll already be comfortable with some of the steps and this will really amp up your knowledge and experience. If you're new to mushroom growing, you can absolutely jump straight in with this kit. Don't let any of the terms you might read here put you off... colonise this, inoculate that and the likes. They're simple really and you'll soon know all the lingo. The grow guide we provide will take you carefully through each step.

What's in the box
  • 1 Sterile Injectable Grain SpawnTub
  • Bag of Substrate (and casing) Mix
  • HEPA-Filtered MonoBox
  • XXXL Cylo Grow Tent (New 2026)
  • Hygiene Kit
  • Perlite (New 2026)
  • Downloadable Grow Guide

For detailed specs, scroll down.

How it works

Simple steps, big yields

1

Inoculate the grain

Working somewhere clean and draught-free, fit a sterile needle to your gourmet culture or spore syringe and pierce through the rubber port on the SpawnTub lid to add 3ml of solution to the grain. To speed up colonisation, move the needle around when distributing the solution throughout the grains.

2

Colonise the grain

Keep the SpawnTub somewhere clean and warm. Aim for around 24 degrees, though anywhere from 18 to 24 degrees is fine. Just keep it consistent and watch out for condensation. Never use a heat mat and don't open it up until your grain is fully colonised (completely covered in healthy, white mycelium). This will take 2 to 3 weeks on average.

3

Prepare the substrate

Add water to the bag of substrate mix and pop in your microwave (full details can be found in the guide, including if you don't have a microwave). Leave to cool, then mix with your colonised grain. You'll need to carefully break up your grain as it will be a solid block when you take it out of your SpawnTub. For efficient colonisation of your mix, you need individual mycelium covered grains spread evenly throughout your substrate.

4

Colonise the substrate

When you're happy with your mix, add it to your 3.1L MonoBox. Secure the lid and again, keep it somewhere clean and warm. Colonisation takes around 10 days on average, but give it an extra few days when it looks finished, to make sure the centre is full of mycelium too.

5

Fruit your mushrooms

Put the colonised substrate, now called a cake, into your grow tent. Drop the environment temperature slightly to around 20 to 22 degrees. Follow our instructions and advice on creating the right conditions, and you'll start to see your first mushrooms forming in around 3 to 7 days. Dunk your cake in water after you harvest all the mushrooms and get ready for your next flush. The first flush is the biggest.

What we cover

The Sterile Guarantee

We guarantee the kit, not the grow.

The SpawnTub in the MonoBox Grow Kit will arrive sterile. If this shows contamination on arrival, before you have used it, get in touch with photos and we will replace it. What happens after you use them though, comes down to your own technique and conditions. We don't guarantee success and no reputable seller can promise that to you. We stand by providing you with sterile SpawnTubs, the rest is down to you (with our instructions and troubleshooting guides for support!).

At a glance

The spec sheet

Kit type
Easy two-stage, grain spawn into bulk substrate
Vessel
HEPA-filtered grow box (3.1 Litres capacity, HEPA filtered), plus a new (July 2026), bigger, badder Cylo Grow Tent to fruit in (51 x 61cm, 800 gauge)
Substrate
1KG Bag of Coir / Verm / Gypsum Substrate mix post-prep
Spawn
Sterile Rye Grain SpawnTub (New style), 1L each in size holding around 1L (no headroom)
Inoculation
Inject the SpawnTub's rubber port, then shake and leave alone
Solution needed
Clean 3ml gourmet spore or culture syringe, not included
Colonisation temp
Around 24 degrees, 18 to 24 is fine if kept steady
Humidity
2L of perlite for extreme humidity levels (July 2026)
Fruiting temp
A little cooler, around 20 to 22 degrees
Time to harvest
About 4 to 6 weeks from inoculation to the first flush
Yield
800 to 1000g, over multiple flushes
Made
United Kingdom, fresh to order
Suited to
Just about any mushroom species you can think of

What customers say

Reviews

★★★★★ 4.9 from 350 ratings · 308 written reviews ✓ All from verified purchases
★★★★★✓ VerifiedReviewed 4 Jun 2025

A great place to start if you're interested in growing your own medicinal or gourmet mushrooms. Works every time.

1
★★★★★✓ VerifiedReviewed 15 Jan 2024

Great gear. Perfect

1
★★★★★✓ VerifiedOrdered 5 Apr 2021 · Reviewed 2 Jul 2021

Professional kit

1
★★★★★✓ VerifiedOrdered 10 Apr 2023 · Reviewed 17 Jun 2023

Made things easy for a first time newbie. Followed all the steps carefully - in particular making sure the temperature range was right. I found each stage took a bit longer than expected but played the long game and let things develop as they needed to. The final stage in particular seemed to take ages with no action and no sign of any actual mushrooms developing despite a vigorous web of mycelium evident. But all good things come to those who wait and, hey presto, just as I was giving up hope that anything would happen, 2 months later I have harvested my first flush - over 200g of fine mushrooms. Now hoping for a second flush..

Reply from Cylocybe

Love this review, Rupert. Most folks never get around to leaving one after seeing results, so it's VERY appreciated that you put the effort into writing this. Regarding duration it takes - it changes wildly on numerous factors, especially for gourmet mushroom spores because there's a TONNE of genetics with different traits - a lottery. That's when things get really fun though... strain isolation and cloning for characteristics you want like speed, look, size, etc.

1
★★★★★✓ VerifiedOrdered 16 Jun 2022 · Reviewed 7 Jul 2022

thanks Cylocybe, good instructions with this kit

1
★★★★★✓ VerifiedReviewed 1 Sep 2025

Great piece of kit, highly recommended

1
Showing 1 to 6 of 308

For cultivating legal gourmet mushroom species only.Sold as a sterile substrate kit for home cultivation. We trust you to be responsible.